Development of sensitive automated pH meter for real-time biosensor applications
نویسندگان
چکیده
A computer controlled pH meter was developed using simple op-amp circuits. Analog to digital converter (ADC) card was used to acquire the output potential from the op-amp. S9ftware based signal averaging helped in reduction of electrical noise. The instrument was calibrated against pH and ammonia selective electrodes where it followed Nemstian behaviour and was l\Sed in the development of a simple biosensor for urea. The enzyme urease was immobilized in polyvinyl alcohol • polyacrylamide composite polymer gel. The enzyme membranes were used in conjugation with the ammonia selective electrode connected to the fabricated instrument. The studies with this biosensor assembly yielded linear response behaviour with a response time of 180 sec.
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